FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923033
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21789811.330954799007609No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC937384.874487333290692No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC824784.28895395970844No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC668643.477007414849355No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC631283.282730977575528No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC490392.5500862439698126No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC479382.492832936304265No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC431412.243383238873176No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC324191.6858265042773577No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC323931.6844744733969723No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC314241.6340853225087664No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCAGGGTTCC218741.137473979905701No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC201261.0465759037936426No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC144650.7521971801835954No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC139830.7271326077087601No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC127460.6628071385150437No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC119060.6191261408410569No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC114790.5969216336901135No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT109820.5710770433996712No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCAGGGTTCC92270.479814958973663No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG72530.3771646144397938No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC72120.3750325657438016No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC54140.2815344302463868No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG51110.2657780703711273No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCGTGGTCCC48470.2520497568164457No Hit
AAGACCGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGTGCC46540.24201352758897013No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC46450.24154551689960596No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGTCCC39230.20400065937506012No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC36480.18970033275560014No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC36470.189648331567893No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC31800.16536377690866458No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC31050.16146368783063006No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGGGTTCC30720.15974764863629487No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC29990.15595156193367454No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC29630.15407951917621798No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCATGGTCCC27410.14253525550523574No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC27330.14211924600357873No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC25390.13203101558839603No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCGTGGTCCC23330.12131877092072783No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22450.11674266640250062No Hit
AAGACGGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGTGCC21960.1141946082048514No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACCGTACT20330.10571841460858968No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19660.10223433503221213No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19480.10129831365348384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)