FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804765
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60626515.934361254900105No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC46039612.100510806843525No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1342443.5283125239009507No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1282463.3706680964527376No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC1212663.1872139277984317No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC1204933.166897298519094No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT616581.6205468668892824No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC552031.4508911851323276No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC466571.2262781012756372No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG354050.9305436735251718No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC351420.9236312886604034No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG330400.8683847754066283No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC273470.7187566117749716No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC259230.6813298587429184No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC99840.2624077965393395No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC72500.19055053334437214No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC66910.1758584301527164No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC64840.17041788389033224No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC62380.16395230717271633No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC58810.15456933608251758No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC52010.13669701019642475No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC50400.13246547421457042No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC49900.1311513326052989No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC49490.13007373648569623No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC49140.12915383735920613No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC48150.12655183697284852No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTAGCACGGTGAGCC42410.11146549129841132No Hit
AGCGACCTCGGATGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC41890.11009878402476894No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCGCGGTGAGCC40340.10602494503602719No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC38590.10142544940357683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)