FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209209
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC33100110.314099206377646No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC2984599.300079863916624No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG1446824.508338347549193No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1381274.304082407845672No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC843172.6273452430178277No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG698232.175707471841192No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC669552.086339655659697No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC532271.6585706945231675No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC523401.630931484985864No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC376651.1736536947266445No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC233640.7280298665496701No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC233410.7273131790419384No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT224400.6992377249347114No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC211560.6592278658074311No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA202580.6312458926794734No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC104570.32584353340651856No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGCAGAG103750.32328838663982307No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGCAGAG55330.17241008609909794No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC55050.17153759695925072No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC54070.1684838849697854No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC44390.1383206889922096No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC39310.12249124316926693No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGCG38780.12083974586884182No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC38710.12062162358388001No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC36950.1151374061334117No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC35810.11158512892117652No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC33610.10472985710809112No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC33370.10398200927393636No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC33100.10314068046051222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)