FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3486876
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC48741513.97855845748458No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC42783912.269980349172153No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC41595411.929130832298023No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC1816905.21068142371567No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC1237933.550255300159799No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC948362.7197984671665987No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG170850.48998014268359413No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC108850.31217055037231034No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC57600.165190847050483No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC55850.16017202791266452No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC55580.15939769581711538No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC54410.15604225673640243No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC52230.1497902420390057No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC50510.14485745980069265No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC45700.13106287691331725No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC45270.129829681353739No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC44620.1279655485311207No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC43660.125212367746946No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGTGAGCC43200.12389313528786225No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC42560.12205768143174578No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC41950.12030826447513476No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC40720.11678075159541092No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC40480.11609245639936723No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGGACCGTGAGCC39630.11365474424671254No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCGGGAGCC37230.10677179228627573No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCAAGCACTGAGAGCC37160.106571039520763No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC36640.10507973326266835No Hit
AGCGACCTCGGATGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC36180.10376050080358463No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC35000.1003763827563699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)