FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358632
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC60413113.860564507395898No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC4113949.438603671977813No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC2588445.938652311092104No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC2025284.646595537315378No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC1902874.365750538242274No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1386753.1816175350431055No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC1368463.139654827477979No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC832201.90931466570245No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT588681.3506072547533263No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG343660.7884583970383368No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC318140.7299079160617369No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC222200.5097929809169482No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTGTGACCGTGAGCCTG209470.48058656936396554No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC105510.24207136551101355No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG76790.17617913143389946No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCAGAAACTCCCGCTA76010.17438957911564912No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC74060.16991569832002335No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC73100.1677131723898691No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC65470.1502076798408308No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC62680.14380658885632006No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC61130.14025042719825853No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCGGGAGCC58350.13387227919218692No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC58010.13309221792525727No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCCGGAGCC51590.11836282576735085No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC50190.11515080878587594No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC49510.1135906862520167No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC48400.11104401564527586No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGGCCGTGAGCC47770.10959860800361214No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC46720.10718959526750595No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC44580.10227979788153714No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGGACCGTGAGCC43660.10016904386513933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)