FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927943
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC69722617.75041032927413No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC46056811.725424732487207No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC2130865.424875055467964No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC1440973.6685104646375977No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1326383.3767801620339197No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1098042.795458080730805No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC999832.5454289942598454No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC484361.2331136169745844No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC332800.8472628039663508No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA126150.32116046490491335No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC86120.2192496174206194No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC72960.18574607625416154No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC72480.18452406259459467No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC68880.1753589601478433No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC56860.14475770142285668No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTAGCACGGTGAGCC55770.14198271207092364No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC54580.13895313653991415No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC54450.1386221745071148No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC53590.13643273336705752No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC51000.12983895132897805No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC49800.1267839171800609No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC48620.12377980026695908No Hit
AGCGACCTCGGATGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC48010.12222682457459287No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCGCGGTGAGCC45660.11624404936629681No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCCGGAGCC42850.1090901777342492No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTGTGACCGTGAGCC40250.10247093707826209No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCGGGAGCC39590.10079066829635766No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG39350.10017966146657423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)