FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958955
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60046115.16715901039542No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC3633809.178684779190469No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC2910527.351738021775948No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC1585514.004869972000187No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC1537313.883120671995514No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1122052.8342075118307735No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC977502.4690859077711163No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC570241.440380100304247No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT321190.8112999516286495No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC249390.6299389611652569No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC244620.6178903271191514No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG237890.6008908916620673No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA133680.3376648635814249No Hit
AGCGACCTCGGGTGGGAACACCTTATTCAGGTCCTCTACAACTGTGAGTC113380.28638870611057715No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC108200.2733044452387057No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC106690.2694903074169825No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC69800.17630915228892474No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG68450.17289916152115897No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC64980.16413422228845745No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60240.15216136581496884No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC59460.15019114892692642No Hit
AGCGACCTCGGGTGGGAACACATTGTTCAGGTCCTCTACAACTGTGAGTC51140.12917550212114057No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC47560.12013271178884326No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC46200.1166974618302052No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC45810.11571235338618399No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC41860.10573497299161017No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTAGCACGGTGAGCC41580.10502771564718469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)