FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757085
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG23422213.330146236522422No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG1677559.547346884186023No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1167806.646235099611003No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC907015.162015497258244No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC713204.058995438467689No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC458552.609720076148849No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC445512.535506250409058No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC299621.7052106187236244No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC174400.9925530068266476No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGCAGAG174100.990845633535088No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGCAGAG115590.657850929237914No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAC79660.4533645213521258No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGACCGT66800.38017511958727096No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGCG47690.2714154409149244No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAC41570.2365850257671086No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAT40800.23220276765210562No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC36750.20915322821605103No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCACCAGCACGGTCAGCC35750.20346198391085235No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGCG34350.1954942418835742No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGCC31780.18086774401921366No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAGCTT31300.17813594675271827No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC31240.17779447209440635No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC30590.17409516329602723No Hit
AGCGACCTCGGGTGGGAACACATTGTTCAGGTCCTCTGGAAAGGGAAGAG29470.16772096967420472No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAT28660.1631110617869938No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAGCTT28170.16032235207744647No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG24740.14080138411061502No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGGAGAG23840.1356792642359362No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTGGAAAGGGAAGAG20860.1187193562064442No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGCAGAT20420.11621520871215678No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGCAGCG20170.11479239763585712No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTCGTA19850.11297119945819355No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAACGGGAAGAG18900.1075645173682548No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGCC18080.1028976970379919No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGACCGT18040.10267004726578395No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG17910.10193018550610812No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG17900.10187327306305613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)