FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434559
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC35513314.587159317149432No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC2389559.815124628320776No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC2136048.773827210595432No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1567996.440550424121986No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC984734.044798257097075No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC901883.704490217735532No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC384011.5773287893207766No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC53590.22012200156167916No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC44210.1815934631282298No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC36120.14836362560940194No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC35970.14774749759607386No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC35790.14700814398008016No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC35770.1469259935783031No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC30380.1247864602993807No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC30020.12330775306739332No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTAGCACGGTGAGCC28350.11644819451900736No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC27940.11476411128257727No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC27740.11394260726480648No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC27540.11312110324703571No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC25870.10626154469864973No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC25130.10322197983289787No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTGAGCC24680.10137359579291362No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGTGAGCC24480.10055209177514285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)