FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417101
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC63816611.780581532446968No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC3473316.411750491637501No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC3164995.842589975708409No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC2999185.5365037498839325No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT2219434.097080707928466No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC2087003.85261415653871No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC1875703.462553125740133No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC1818863.357626154653568No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1653023.0514845486543445No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC1106892.043325387508928No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCACGACAGAGAGCT110330.2036698226597584No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC81950.1512801773494716No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTGAGCC69970.12916502756732798No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC69310.1279466637229027No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC67030.12373777044216085No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC64510.11908583576344617No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC56210.1037639874168859No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC55860.10311788537817551No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGCC54520.10064423757282724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)