FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439408
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC64218911.806229648520574No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC4309897.923454170012619No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC4102847.542806128902263No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC3666426.7404761694655No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC3391976.2359175851489725No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1321412.429326867923862No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC1301122.3920250144868707No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC1155142.123650220759318No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC565551.0397271173627718No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC408420.7508537693807856No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT342720.6300685662851546No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC308020.5662748593229263No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCCGCCGAATGGC198940.36573833034771436No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC164800.3029741471866056No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTACCC138030.2537592326223736No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA84940.15615669940552354No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC78190.14374726073131489No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCGGGAGCC77350.14220297502963558No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCCGGAGCC68950.12676011801284257No Hit
AGCGACCTCGGGTGGGAACACATTGTTCAGGTCCTCTACAACGGTTAACC68310.12558351938299167No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC66010.12135511805696501No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60290.11083926780267264No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60200.11067380862034987No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC57850.1063534855263661No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG54400.10001088353732612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)