FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027627
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC71549814.231326230048492No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC51793510.301778552784445No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC2006133.9902124799632115No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC1952623.8837805588998546No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC1587693.1579311671291443No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC1540093.0632542947199544No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC1348022.6812251585091733No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1264352.514804698121002No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC781531.5544709263435812No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC724281.4406001081623596No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC611881.2170353926414987No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC91760.1825115506778844No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT79580.15828540979670927No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC75400.14997134831203668No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC75220.14961332652561535No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC73370.14593365816517415No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA68110.1354714659619737No Hit
AGCGGCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC67210.13368135702986716No Hit
AGCGACCTCGGGTGGAAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60860.12105114400889326No Hit
AGCGTCCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC60250.11983784795490994No Hit
AGCGACCTCGGGTGGGAACACGTTATTCAGGTCCTCTAGCACGGTGAGCC59460.11826653011450532No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC56300.1119812587528868No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC56250.11188180825665865No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC53100.10561642699428578No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTTCCATTTCCACACTT52080.1035876368712317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)