FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760197
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20829011.833334564256159No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC884355.0241535464496305No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC496432.8203093176502403No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC461332.6208998197360867No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC455552.588062586176434No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC434342.4675647100864277No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC389972.215490652466741No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC299231.6999801726738544No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC272561.5484630413527576No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC207721.1800951825278647No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC193111.097093109464452No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC165980.9429626342960475No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC165520.9403492904487396No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC128450.7297478634493753No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC124890.709522854544122No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC123530.7017964466477331No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC117070.6650960091398861No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC111240.6319747164663955No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC100930.5734017271930357No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGTCCC86280.4901724068385527No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT77890.44250728753656554No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC73130.41546485989920445No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGTTCC71490.40614772096532376No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC57400.3260998626858244No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC50380.28621796310299363No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCG49720.28246838280033426No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC46990.26695875518478895No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG44700.25394884777101656No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC43070.2446885206599034No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATCCC42090.23912096202868202No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGACGAGACGGTGAGCAGGGTTCC41780.2373597955228875No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC38700.21986175411047743No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC35880.20384082009002402No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC35840.20361357279895376No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35350.20082979348334307No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATTACCAGGGTTCC33290.18912655799322461No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCACGCTGCT32970.18730857966466255No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC29420.16714038258217687No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC29360.1667995116455715No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC29230.16606095794959314No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC29190.1658337106585229No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC27560.15657338354740977No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC25900.14714262096799394No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGTTCC25620.14555188993050208No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGGGGTCCC24690.14026839041311853No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGATTCC24540.13941621307160507No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC24460.13896171848946454No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC24140.13714374016090244No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA23430.1331101007444053No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT23370.13276922980779993No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC22230.12629268201229749No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21840.12407702092436243No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA21240.12066831155830852No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCG20840.11839583864760592No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC20310.11538481204092498No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC19590.11129436080166027No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19490.11072624257398461No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19170.10890826424542253No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC19020.10805608690390904No Hit
AAGACGGATGGGCCCTTGGTGGAAGCGGAGGAGACGCTGATCAGGGTTCC19000.10794246325837391No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18780.10669260315748748No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG18770.10663579133471993No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATCCC18440.10476100118339025No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGTTCC18180.10328389379143357No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC18080.10271577556375791No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCT17650.10027286718475262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)