FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875180
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1392477.425793790462783No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC952935.081805480007253No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC761184.059236979916594No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC610393.2551008436523428No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC594163.1685491526146823No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC555942.9647287193762732No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC426082.2722085346473406No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC421192.2461310380870105No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC401212.1395812668650476No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC265271.414637528130633No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC265031.4133576509988375No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC208851.1137597457310764No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC205141.0939749784020734No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT184140.9819857293699805No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC166620.8885546987489201No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC138280.7374225407694195No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC115330.6150342900414894No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC95190.5076312673983298No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC92020.4907262236158662No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC82470.4397977794131764No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC79970.42646572595697485No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC76550.4082274768288911No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC73570.39233566910909884No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC70640.37671050245843063No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC62380.3326613978391408No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG61560.32828848430550667No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC58910.31415650764193304No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGTTCC53000.2826395332714726No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC42190.22499173412685716No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC41600.2218453695111936No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC41070.21901897417847885No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCG36900.19678110901353468No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGCTCC36390.19406137010846958No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC35330.18840857944304015No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG35030.18680873302829595No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTACT33900.18078264486609286No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC32510.1733700231444448No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGTCCC30270.1614245032476882No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGAGACCAGGTTTCC28440.15166544011774868No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA26630.1420130334154588No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGTTCC26570.14169306413250995No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC26140.13939995093804328No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA25530.1361469298947301No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC25520.13609360168090529No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC24720.13182734457492082No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23650.12612122569566653No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21400.11412237758508517No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC19950.10638978658048827No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC19880.10601648908371464No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC19320.10303010910952548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)