FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960417
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1249136.371756621167843No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC790644.033019505543973No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC718023.662588112631139No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC695703.548734784487178No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC549972.8053725304361268No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC427462.1804544645348414No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC414862.116182424453573No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC387331.9757531178315635No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC361481.843893416553723No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC313791.600628845801684No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC283991.4486203700539222No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC225891.1522548519014066No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC201011.0253430775187116No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC197931.0096321343877348No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC195420.9968287359271012No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT145240.7408627858256687No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC118630.6051263583207042No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC104780.534478123786929No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC97310.49637398573874847No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC95570.487498323060859No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC87990.44883307990085786No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC85070.43393828965980197No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGTTCC84730.43220396476872014No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC75880.38706050804497205No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC64780.33043990130671175No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC59830.3051901712747849No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG58950.3007013303802201No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG57600.2938150403715128No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGAGACCAGGTTTCC54030.2756046290151534No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC48170.2457130294218016No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC48030.24499889564312083No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC44370.22632939828618093No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC35460.18087988422871257No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA33510.170933020882802No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC32840.16751538065625834No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGCTCC32720.1669032659888177No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGATCAGGGTTCC32690.1667502373219575No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC28880.14731559663071683No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC27670.14114344040069027No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGTTCC25870.13196172038908047No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC25770.13145162483287995No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGAGACCAGGTTTCC24360.12425927749045228No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC23750.12114769459762896No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC23510.11992346526274768No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC23440.1195663983734073No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGATTCC22770.11614875814686366No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22230.11339424214338072No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC21860.11150688858543871No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC21670.11053770702865767No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC21490.10961953502749669No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC21070.10747713369145442No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC20940.10681400946839371No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCG20340.10375343613119045No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC20250.10329435013060996No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGTCCC20120.10263122590754925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)