FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906663
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1259046.60336934214384No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC735863.859413016353703No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC556972.9211769463193025No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC556562.919026592533657No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC546192.864638376052821No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC540412.834323632440552No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC495202.597207791833166No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC477002.5017530628118343No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC328541.7231152017949687No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC292911.536244213057053No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC249241.3072053110591648No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC239731.257327592762853No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC222291.1658588853929615No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC215861.1321350443156446No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC214981.1275196508245033No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC173820.9116451098070294No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC125820.6598963739265933No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC118370.6208228722118172No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG104210.5465569951270886No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC83710.43903930584481893No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT74510.39078746480106863No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC69810.3661370677461093No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG69310.36351468508068807No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC63810.3346684757610548No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC57430.30120687295028015No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC55340.29024531340881954No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC54810.28746558778347303No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGTTCC53160.278811724987583No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC53040.2781823531478819No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCCGGATTCC47850.2509620210808098No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC45870.24057738572574178No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC45330.23774521244708688No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC44440.23307737130263714No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCG43020.2256298045328409No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC42760.22426616554682188No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC40420.2119934146726506No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC39710.2082696312877525No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC37020.19416121254778637No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGTCCC36770.19285002121507575No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC36570.19180106814890727No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC35840.1879723894573923No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC33320.17475558082366943No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC31550.16547234618807832No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGTAGGGTTCC31540.1654198985347699No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC29640.15545484440616933No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGCGTTCC29470.1545632342999261No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGTCCC27230.14281495995883908No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCACGCTGCT26850.14082194913311896No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA26410.1385142523875483No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC23870.12519254844720856No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23040.12083939322260935No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACTAGGGTTCC22350.11722050514432808No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGTTCC21950.11512259901199111No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGCTGACCGGGGTTCC21900.11486036074544899No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA21490.11271000695980359No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGAGACGGTGACCAGAGTGCC21170.11103168205393402No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCCGGATTCC21050.11040231021423293No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTCGGGCTCC20710.1086190900017465No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC20570.10788482285542857No Hit
AAGACCGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACAGGCGTTCC20530.10767503224219488No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC19690.10326942936428724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)