FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997129
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC20117510.07321009308863No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC1055275.283935088820002No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC789573.953525285547403No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC745263.7316567933268208No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC454172.274114491352336No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTGCC424502.1255512287889267No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGTGGTCCC409312.0494920458317916No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC399772.0017234740469942No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC324921.6269354658612438No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC301471.5095169115264964No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC232541.164371455223974No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC225601.1296215717662705No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC192610.9644344456467259No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTGCC189360.9481610852378589No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTGCC187790.9402998003634218No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC185040.9265300338636113No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC171750.8599845077608907No Hit
AAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGTGGTCCC165920.8307926027812926No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC146920.7356560342371474No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC127070.6362633560476063No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC114690.5742743708593686No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTAAGGAGACGATGACCAGGGTTCC90040.45084719114288563No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC89580.44854388474655366No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGTCCC89570.44849381286837253No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTGCC83390.4175493921524348No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC78010.3906107216909874No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT73640.36872931092583405No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC70340.3522055911260615No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC68210.34154028107348094No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC68080.3408893466571263No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC62180.3113469385302602No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC60920.3050378818794379No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC54750.2741435330416813No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC48010.24039508714760038No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC47260.2366396962840157No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC43580.2182132451133602No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC42070.21065239150800974No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC39440.19748348754637285No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTAAGGAGACGATGACCAGGGTTCC38180.19117443089555056No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGTCCC37760.18907141201194314No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTTACCGTGGTCCC35850.17950768327934752No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACAGTGACCAGGGTGCC35680.1786564613502683No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC35630.17840610195936266No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGCTGACCGTGGTCCC35100.17575229241576285No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC31160.156023972412398No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC30930.15487231921423203No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC30060.15051606581247381No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCGTGACCAGGGTGCC29940.14991520327430027No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC29190.1461598124107156No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC28580.14310542784166672No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGTCCGTAC26730.13384213037815784No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTCTTCC26000.13018688327093542No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGCTGACCGTGGTCCC25940.12988645200184865No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC24180.12107380144196994No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC23860.11947150134017381No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGCCGTACT23200.1161667573802193No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC22550.11291208529844593No Hit
AAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC20200.10114519392588059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)