FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652820
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19319311.68868963347491No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC834245.0473735797001495No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC356292.1556491329969387No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC308381.8657809077818517No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTGCC306661.8553744509383963No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGACCAT270541.6368388572258321No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC261691.5832940066068901No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC252691.5288416161469487No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACAGTTCC252611.528357594898416No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC152600.9232705315763362No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTGCC145690.8814631962343146No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC144790.8760179571883205No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC143840.8702702048619935No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC141260.8546605195968103No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCTTCC136460.8256192446848417No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC122040.7383744146368025No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGCTGACCAGGGTTCC121270.7337157101196743No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGACCAT118770.7185900461030239No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGTTCC116600.7054609697365715No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC116160.702798852869641No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTCACCGTGGTCCC113810.6885807286939897No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACAGTTCC107940.6530656695828947No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC106540.6445952977335705No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC98260.5944990985104246No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC97800.591715976331361No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC88150.5333309132270907No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT75640.45764209048777243No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC74900.45316489393884396No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC72030.4358006316477293No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGTTCC68340.4134751515591535No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC65160.3942353069299742No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC62640.3789886376011907No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC61990.3750559649568616No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACGAGGGTTCC54800.3315545552449753No Hit
AAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGCTGACCAGGGTTCC54350.3288319357219782No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGCCCC52090.3151583354509263No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTCACCGTGGTCCC51050.30886605921999977No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGTTCC47280.286056557882891No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC42720.25846734671652083No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCATTGTCCC34820.2106702484239058No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTTTTAAAATAGCAGCAAGGAAAAT33730.20407545891264625No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC32780.19832770658631915No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC32670.19766217736958652No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC32510.1966941348725209No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGTTCC28900.1748526760324778No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTGCC27710.16765285996055226No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC26740.16178410232209195No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26520.1604530438886267No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACGAGGGTTCC25510.15434227562589997No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGCCCC24540.14847351798743966No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGATCC23130.13994264348204888No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC22470.13594946818165318No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAACTGAAGAGACGATGACCAT22020.1332268486586561No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20680.12511949274573153No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC19270.11658861824034075No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC18970.11477353855834271No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGATACGGTCACCAGGGTTCC18710.11320046950061109No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCCGAGACGGTGACCAGGGTGCC18660.11289795622027807No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC18650.11283745356421147No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17640.10672668530148474No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17610.10654517733328495No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17320.10479060030735349No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT16630.10061591703875801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)