FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442965
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1131257.839760493151254No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC507083.5141531499378016No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC337302.3375480347756183No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC305472.1169605638390396No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC150971.0462485230064484No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC147551.0225473244326786No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC143720.9960047541000648No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC123870.8584407799218969No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC121800.8440953176272467No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC121360.8410460406177558No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC120220.8331456410931658No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC114570.7939901522212944No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC113170.784287907191096No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC101690.7047294979434706No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC98070.6796422643653866No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC97500.6756920646030916No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC66650.46189616518765186No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACTGTCCC57900.4012571337489128No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC57530.39869296899093193No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGTTCC56780.39349533772475426No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC55170.3823377559400263No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGTCCC52950.3669527673921405No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC52080.36092351512337445No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC51620.35773563461345215No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC48780.3380539375521929No Hit
AAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC46060.31920386149352203No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC45990.3187187492420121No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC44050.305274209700166No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGAGTTCC42820.29675009442363465No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGTTCC41380.28677064239257366No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC41360.2866320388921422No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGATCCC38770.2686828855862755No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC33650.2332003894758362No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC32890.2279334564594429No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC32290.2237753514465008No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG31850.2207260744370099No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC29770.20631131039214398No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC27410.18995609734123836No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGTCCC27260.1889165710880028No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGACACGGTGACCAGGGTTCC27180.18836215708627724No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC26780.18559008707764915No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACTGTCCC26300.18226360306729547No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC26140.18115477506384425No Hit
AAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGTCCC25410.17609574729809802No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC25220.17477901404399968No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGTTCC24980.17311577203882283No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT24670.1709674177821361No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC24660.1708981160319204No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC23150.16043355174934942No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGCGACGGTGACCAATGTCCC22800.15800799049179987No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC21030.14574158070362067No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGATTCC20940.14511786495167933No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGGGTTCC20570.1425537001936984No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC20560.14248439844348268No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC20480.14192998444175708No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC20450.14172207919110996No Hit
AAGACCGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGTTCC20290.14061325118765874No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19590.13576212867255963No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGAGTTCC19520.1352770164210497No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC19350.1340988866673828No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC18620.12903985890163655No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGATCCC18530.12841614314969527No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGTTCC18260.1265449958938713No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGTTTCC18090.12536686614020437No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGAAGGTGACCGTTGTCCC17750.12301060663287053No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA17660.1223868908809292No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGTTCC17450.12093155412639947No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC17240.11947621737186973No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATCGTCCC17210.11926831212122262No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17170.1189911051203598No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTTCC17050.11815948411777139No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC16590.11497160360784911No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC15850.1098432740918872No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGGTTCC15830.10970467059145579No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGAGACCGTGGTCCC15770.10928886009016157No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGTTCC15410.1067939970823963No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC15400.1067246953321806No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC15270.1058237725793765No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC15170.10513075507721947No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC15150.10499215157678808No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC15070.10443773757506246No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC15030.10416053057419965No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGAGCATTGTCCC14960.10367541832268974No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC14660.10159636581621868No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCAGGGTTCC14610.10124985706514017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)