FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703135
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC903175.3029853769665936No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC801914.7084347394657495No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC753694.425309796346149No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC467962.747638912945832No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC414092.4313398526834336No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC387422.2747462767191093No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC339311.9922672013668912No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC333571.9585646469598712No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC330441.9401867732152764No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC313861.8428368860953475No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC293221.7216486068338681No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC216581.271654918723413No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC180641.060632304544267No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC128160.7524946642515127No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC126200.7409864749417985No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC116950.6866748672301374No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC113860.6685318544918636No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT109630.6436953030734499No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC104540.6138092400191412No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC96990.5694792250761096No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC91250.5357766706690896No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC86430.5074759194074457No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC82210.48269808324061214No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC71040.41711314722555753No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGTCGACGGTGACCGTGGTCCC55560.3262219377794479No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC55470.32569350051522633No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC47590.27942588227004905No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG43880.25764252393380443No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGTTCC42750.25100770050524474No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC36280.21301893273287204No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC30550.1793750935774322No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC29010.17033294483408537No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC26470.15541927093272115No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC25260.14831472549152006No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGTCGACGGTGACCGTGGTCCC24330.14285420709456387No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC24180.14197347832086124No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGGCGTTCC23620.13868542423237148No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC22040.12940841448270396No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCGTGACCAGGGTGCC21020.12341945882152619No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCTGGGTTCC20850.12242129954466321No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG19760.11602133712242423No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGTCCC17630.10351498853584715No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC17590.10328012752952644No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGTTCC17560.10310398177478591No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)