FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979761
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1040015.2532098571494235No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC941224.7542102304268035No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC916174.62767980579474No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC846724.276879886006442No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC709093.5816949621696756No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC412022.0811603016727775No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC392441.982259474754781No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC337571.7051048081056248No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC321661.6247415723413077No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC289971.4646717457309242No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC251391.269799738453278No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC179060.9044526081683596No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC178390.9010683612819931No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC159090.8035818464956124No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC157850.7973184641984562No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC157350.7947929068205708No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC111790.5646641185476429No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC111450.5629467395306808No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT109600.5536021772325044No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC86450.43666887063640514No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC86040.434597913586539No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGTTCC78030.39413848439281307No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC72780.3676201319250152No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCTGGGTTCC68290.3449406266716033No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC68160.34428398175335306No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC67240.3396369561780437No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC63380.32013965322076754No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGGCGTTCC58250.29422743452366223No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC54200.27377041976278954No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG54190.2737199086152318No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC49870.251899092870301No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG40710.2056308817074384No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGTCCC40180.20295379088687976No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC35070.17714259448488984No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGTTCC34110.17229352431934966No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGTTCC33660.1700205226792527No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC32670.16501991907103938No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC29850.1507757754597651No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGTCGACGGTGACCGTGGTCCC29380.1484017515245527No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC29070.1468359059502637No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCTGGGTTCC28420.14355268135901253No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC26520.13395556332304756No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC26210.13238971774875857No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGGCGTTCC24080.12163084331896629No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTTTTAAAATAGCCAAAAGGAAAAT22600.11415519348042516No Hit
AAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGTCCC21100.10657852134676864No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACACGGGTGCC20920.10566932069072985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)