FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158070
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC683625.903097394803423No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC679995.871752139335273No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC544714.703601682109026No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC436503.769202207120468No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC314622.7167615083716874No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC305972.6420682687575017No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC260982.253577072197708No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC214311.8505789805452175No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC209711.810857720172356No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC202141.7454903416891896No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC176071.5203744160542974No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC150801.3021665357016414No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC82860.7155007901076792No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC72610.6269914599290198No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC67210.5803621542739213No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT56450.487448945227836No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC50650.43736561693161896No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC49080.4238085780652292No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGTTCC48480.4186275441035516No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC48200.41620972825476876No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC38990.33668085694301725No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC37670.32528258222732653No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC37390.3228647663785436No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC35760.3087896241159861No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC32900.28409336223198944No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC32280.2787396271382559No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCGGACCGTGGTCCC23700.20465084148626592No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGACGAGACGGTGACCAGGGTTCC23130.1997288592226722No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC22310.19264811280837946No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGTTCC21840.18858963620506533No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC20410.176241505263067No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC19780.1708014196033055No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG19450.1679518509243828No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG18940.16354797205695684No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC18670.1612165067742019No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC18320.1581942369632233No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGATGGTGACCGTGGTCCC18300.15802153583116738No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGCTAGACCTCCAA15970.1379018539466526No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGTTCC15690.13548403809786974No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGATCC15100.13038935470222007No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC14570.12581277470273816No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA14160.12227240149559182No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGCCCC14040.12123619470325628No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTTACCAGGGTTCC14000.12089079243914444No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACTGTGGTCCC13540.11691866640185826No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC13290.11475990225115927No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTTGTCCC13190.11389639659087965No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC12650.1092334660253698No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCGGACCGTGGTCCC12150.10491593772397179No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)