FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688175
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1158886.864691160572808No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC831504.925437232514401No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC576723.416233506597361No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC512403.035230352303523No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC429422.5436936336576483No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC376482.230100552371644No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC352262.0866320138611223No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC203701.2066284597272203No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC194731.1534941578923987No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC183511.0870318539250967No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC161840.9586683844980527No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC160480.9506123476535311No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC150910.8939239119167148No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGATTCC124550.7377789624890784No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC116800.6918713995883129No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC115940.6867771409954536No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC108180.6408103425296549No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC100630.5960874909295542No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC98640.5842996134879381No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTCCC98520.5835887867075392No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC89560.5305137204377508No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC84360.4997112266204629No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC63500.37614583796111184No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGATTCC51780.3067217557421476No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC50150.29706635864172853No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC48750.28877337953707405No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT47400.28077657825758584No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC46880.2776963288758571No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC45650.2704103543767678No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC45480.2694033497712026No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC45350.2686332874257705No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC45320.2684555807306707No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA44600.264190620048277No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG44530.26377597109304424No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC41980.24867090200956654No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG38300.22687221407733205No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC35940.21289262072948598No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC35660.2112340249085551No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC35530.21046396256312289No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC34630.20513276171013076No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCGTGGTCCC33250.1969582537355428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTTACCGGGGTTCC32000.18955380810638706No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC31220.1849334340337939No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGACGACCATTGTCCC29750.17622580597390672No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC26880.15922519880936514No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTAAGGAGACGGTGACCAGGGTTCC23760.1407437025189924No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACACTGACGGGGGTGCC23720.14050676025885941No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC22220.1316214255038725No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGACTCC21790.12907429620744296No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTCCC21380.12664563804107987No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCACGGTTCC21260.1259348112606809No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC20860.12356538865935109No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGCGACCAGGGTTCC20550.12172908614332045No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC20310.12030743258252255No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC20090.11900425015179113No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC20060.11882654345669139No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC19610.11616094303019532No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19460.11527240955469663No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC19380.11479852503443067No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC18980.11242910243310082No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18660.11053356435203696No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC18310.10846031957587336No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGAGCATTGTCCC17390.10301064759281472No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17200.10188517185718306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)