FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620697
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC769664.748944435634791No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC714844.4106949047230914No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC713394.401748136758444No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC708584.372069547855028No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC390992.4124805562051392No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC382922.3626871648432743No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC362642.237555817034276No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC331462.0451694548703427No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC274371.692913604455367No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC228641.4107510534048007No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC219831.3563917252885642No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC169521.0459697278393185No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC162651.0035805582412998No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC113300.6990819382031311No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT110260.6803245764013878No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC100910.6226333484914206No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC80540.496946684050134No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC60260.3718153362411358No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC60240.37169193254507166No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA53600.3307219054517902No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC52740.3254155465210338No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC47630.2938859021766561No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC46820.28888805248606003No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC44380.27383280156623974No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC40700.2511265214904452No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC39500.24372229972659915No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC35090.2165117847444649No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC33140.20447992437821505No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC32160.19843314327107411No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC32160.19843314327107411No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG27370.16887795806372197No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG26930.16616307675031175No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC23850.14715890755644023No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC23100.14253126895403645No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGAGCAGGGTTCC23080.14240786525797233No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC20920.12908026608304946No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC19700.12155264062313931No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC19480.12019519996643419No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC19380.11957818148611368No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC18740.11562926321206246No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC16830.10384421023794084No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC16800.10365910469384469No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC16750.10335059545368444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)