FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512565
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC44563829.462403268619862No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20095613.285776148463041No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC164221.0857054077014872No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC132830.8781771361891886No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC129090.8534509260759042No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC125690.8309725532456456No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC105370.6966312191542182No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC86140.5694961869407265No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC84320.5574636461904117No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC83870.5544885674334656No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC80600.5328696617996582No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC72610.48004548564855065No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC72160.4770704068916046No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC69370.4586249185985396No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGTCCC65540.433303692733866No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC55420.3663974771332141No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC52570.3475553116725562No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC43370.28673147930832726No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC41540.2746328256967469No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT40110.26517868653578525No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC39060.25823683610291126No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC38470.25433617728824875No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35840.23694849477543115No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC35730.236221253301511No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35590.23529567324379447No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGTCCC31610.2089827544601389No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC30430.2011814368308139No Hit
AAGACCGATGGCCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC29020.19185952339238313No Hit
AAGACCGATGGACCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26130.1727529064866634No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGATGACCAGGGTTCC25480.16845557050440807No Hit
AAGATCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC24160.15972867281736652No Hit
AAGACGGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23820.15748083553434067No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC20680.13672139709698428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC20600.13619249420686053No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACCGTACT20330.13440744695269294No Hit
AAGACCGATGGGCCCCTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20160.13328352831118No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC20110.13295296400485268No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT20000.13222572253093257No Hit
AAGACCGATGGGCCCTTGGTGGAGACTGAGGAGACGGTGACCAGGGTTCC18950.1252838720980586No Hit
AAGACGGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18780.12415995345654567No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC18630.12316826053756368No Hit
AAGACGGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17540.11596195865962784No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC17480.11556528149203504No Hit
AAGACCGATGGGCCCTTGGTGGAGCCTGAGGAGACGGTGACCAGGGTTCC17420.11516860432444226No Hit
AAGAGGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC16710.11047459117459414No Hit
AAGACCGATGGGCCCTTGGTGAAGGCTGAGGAGACGGTGACCAGGGTTCC16510.10915233394928482No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGCAGACGGTGACCAGGGTTCC16410.10849120533663016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)