FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608809
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17918711.137866583292361No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC977716.077228558517512No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC686084.264521145766838No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC605463.763405102781001No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC534383.3215875843558806No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC517683.2177840874833494No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC362062.250484675309499No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC339192.108329826598434No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC319471.9857546793932657No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC238201.4805983805411331No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC177351.1023682736732576No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC135360.8413677447105281No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC125050.7772830708928157No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC107850.6703716848923645No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT83710.5203227977963824No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC58610.3643067635747935No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC55480.34485137763401374No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC51720.3214800513920546No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC44350.2756697656465124No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC36820.2288649553800358No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC36380.2261300129474661No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTTTCC34130.21214451187182565No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC32400.20139121548922215No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC31840.19791037966595165No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGAAGACGGTGACCGTGGTCCC31620.19654290844966682No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC30730.19101086580196905No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC28330.17609299798795258No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC28120.17478768455422614No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26910.1672665928646595No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC26560.16509107047511543No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC25080.15589171865647194No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC23740.14756257579364612No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21920.13624985936801698No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21140.13140155232846162No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC20720.12879092546100873No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGTCCC20710.12872876767845032No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTTGATGGCACAGAAGT19700.12245083164005173No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC19080.11859704912143083No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC18510.11505405551560191No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA16660.10355486574229757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)