FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772556
Sequences flagged as poor quality0
Sequence length250
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1691579.543111755002382No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1379937.7849726609483705No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC1247557.0381415312125535No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC801084.519349459199033No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC782974.41718061375776No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC532473.0039671525187357No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC389532.1975610361534414No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC355322.0045629023850307No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC353671.9952543107241747No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC336861.900419507197516No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC323441.8247096283558881No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC215641.2165483065133063No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC140190.7908917969305342No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC133100.7508930606423718No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC118470.6683568812494499No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC117810.6646334445851076No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC104480.5894313071067995No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGCGACGGTGACCATTGTCCC86350.4871496302514561No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC83730.47236871500815775No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC80040.4515513191120619No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTTGGTCCC67800.3824984937006221No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG63190.3564908527572613No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG52950.2987211687529195No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC52050.2936437551197254No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT51800.2922333624438382No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC44730.25234745756974675No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC41170.2322634658651123No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGCGACGGTGACCATTGTCCC37190.20981001446498726No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC34610.19525476204983086No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTTGGTCCC27410.15463545298427808No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC27290.1539584644998522No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCCGTACT24820.14002378486208616No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC24480.1381056508228795No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22100.1246787125484329No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC20150.11367764967651234No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA19620.11068761720363138No Hit
AGCGACCTCGGGTGGGAACACATTGTTCAGGTCCTCTACAACTGTGAGTC19030.10735909048853746No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC18910.10668210200411159No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT18610.10498963079304686No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18090.10205601402720141No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)