FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518972
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1437909.466270609333154No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1267318.34320843307184No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC832895.483247880803596No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC612954.035294923145391No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC591023.8909209649684127No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC529043.482881843773289No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC428162.8187484693595404No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC343172.2592253181757136No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC248181.6338681687351708No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC247471.629193954858944No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC240651.584295168047864No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC233001.5339321593814765No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC149060.9813215780146047No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC148050.9746723441906764No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC102340.6737451381592288No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT95000.6254229834387993No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC90860.5981677081605191No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC76950.5065926165854275No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC67830.44655201017530277No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG44620.29375130022146556No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC38330.2523417153179914No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC36470.24009659164224226No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG28590.18821940101595025No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC24220.1594499437777655No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21580.14206976823799256No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCCGTACT20120.13245800449251205No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC20090.1322605024977419No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC17450.11488032695796895No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC15920.10480772522469145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)