FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823189
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1376257.548586570015506No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1350877.409379938119416No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC1292687.090213905415182No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC771754.232967618826134No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC642783.525580726956997No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC504062.7647161100686763No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC457982.5119721542857048No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC382562.0983013829065444No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC340781.8691424750807515No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC293751.6111878691677055No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC260151.426895401409289No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC247061.355098127511739No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC237711.3038143604420607No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC221281.2136975376661443No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT163390.8961769734240389No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC117210.6428845281536911No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC116190.6372899353824535No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGCTTCC103730.568948145255374No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC94720.5195292424427747No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC52260.2866406061028231No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG39660.21753093069341684No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGCTTCC36980.2028314124317336No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC35540.19493316381351578No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC27330.14990217689992646No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC25360.1390969339986145No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC22380.12275194727480257No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAGCACTGCTGG22110.1212710256588867No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC20280.1112336680399015No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC19720.10816212691059457No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC19690.1079975800643817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)