FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454336
Sequences flagged as poor quality0
Sequence length250
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC1177248.094690635451505No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC970456.672804633867277No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC808115.556556394120753No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC775165.329992518922725No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC441633.03664352666784No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC360972.4820261617672945No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC321152.2082242342897374No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC310872.13753905562401No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC306582.1080410579123394No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC152091.0457693407850732No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC145991.0038258009153318No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC134650.9258520726984685No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC97940.6734344745643372No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC91150.6267465014962155No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT90950.6253713034676993No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC79810.5487727732793523No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC77310.5315827979229009No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC74680.5134989438479142No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGCTTCC46260.3180833039957754No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG38430.2642443011793698No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC37180.25564931350114417No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC35640.24506028868157018No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC29870.2053858255588805No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC26550.1825575382855131No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC24110.1657801223376166No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC19020.13078133251188173No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGCTTCC18230.1253493002992431No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC18220.12528054039781727No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCTGGGTGCC16470.11324755764830137No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC16450.11311003784544975No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC15670.10774676553423693No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAAAGGAGACAGTGACCAGGGTTCC15430.10609652790001761No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC14630.10059573578595317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)