FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463410
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20037713.692471692827027No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC826605.64845122009553No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC428312.9267942681818493No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC405382.7701054386672226No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC395402.701908556043761No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC218681.4943180653405403No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC217521.486391373572683No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC183941.2569273136031598No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC178731.2213255341975249No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC135620.926739601342071No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC113440.7751757880566622No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC86430.5906068702550891No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC83540.5708584743851689No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC75020.5126382900212517No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT70060.47874484935868966No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGATAACCGTGGTCCC68830.470339822742772No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC65700.44895142167950197No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTCCC64130.43822305437300546No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC57650.39394291415256144No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC48360.3304610464599805No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC48110.3287527077169078No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCTGGGTTCC47890.3272493696230038No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC41000.28016755386392056No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35440.24217410021798402No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC34260.23411074135068094No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC33520.2290540586711858No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC31840.21757402231773734No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTCCC30440.2080073253565303No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC30420.20787065825708448No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCTGGTTCC29780.2034973110748184No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA28200.19270061021859902No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGATAACCGTGGTCCC28040.1916072734230325No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC26730.1826555784093316No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC26430.1806055719176444No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC24830.1696722039619792No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC24740.16905720201447305No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC22760.15552715916933738No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGTTCC21830.14917213904510698No Hit
AAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCTGGGTTCC21070.14397878926616603No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCGTCAGCC20710.14151878147614133No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGATGACCAGGGTTCC20060.13707710074415236No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19700.1346170929541277No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGTTCC19560.133660423258007No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC19530.13345542260883825No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19350.13222541871382593No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC18790.12839873992934311No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18720.12792040508128277No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18190.12429872694596866No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG17350.11855870876924443No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC17020.11630370162838849No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC15980.1091970124572061No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC15690.10721533951524179No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC15300.10455033107604841No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC15250.10420866332743388No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC15080.10304699298214444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)