FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598892
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC663314.148560378061808No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC660844.133112180184778No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC617983.8650515481971266No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC443122.7714192078014026No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC413502.5861659198995306No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC281961.7634712038086375No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC280301.7530890141422937No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC254371.5909142080891017No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC202451.2661893361152599No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC192381.2032082216935227No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC187491.1726245424956783No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC171981.0756198667577297No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCAGGGTTCC161811.0120133192235623No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC155030.9696089542007841No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGCTGACCAGGGTTCC136590.8542790882686261No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT122420.7656552162372443No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC116270.7271910798227772No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGACGAGACGGTGACCGTGGTCCC109940.6876011638059356No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC107090.6697763201016704No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC87880.5496306192037986No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC79510.4972818676933777No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC73560.4600685975037714No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGACTCC71220.44543346267290096No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCAGGGTTCC69460.43442583989412664No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGCTGACCAGGGTTCC55580.34761572388879297No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC48190.3013962168801895No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC41500.2595547416585986No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGGGATTCC40630.25411347358045444No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC40060.2505485048396014No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC39000.24391891384783962No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC37380.23378689742646783No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC35400.22140332180034675No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC32990.20633038379077512No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGACTCC30390.19006912286758582No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGACTCC30250.1891935165101833No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGGGGAGACGGTGACCGTGGTCCC30130.18844299677526688No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGTGACGGTGACCATTGTCCC28740.17974947651248488No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC27600.1726195390307788No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAAGCTTCC26180.16373838883426772No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACTGTCCC25460.15923527042476918No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC23690.1481651043347518No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC23690.1481651043347518No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC22980.14372452923649628No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGCGACCATTGTCCC21790.13628187519857501No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC21420.13396777268258267No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC20950.13102823705416No Hit
AAGACGGATGGGCCCTTGGTGGAAGCGGAGGAGACGGTGACCGTGGTCCC20750.1297773708292993No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGGGACGCTGACCGTGGTCCC19350.12102130725527428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC19080.1193326378517123No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC19010.11889483467301105No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGCTGACCAGGGTTCC19000.11883229136176804No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC18550.1160178423558314No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC18130.11339102328362391No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC17640.11032640103271515No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGGGATTCC17360.10857518831791015No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGACGAGACGGTGACCGTGGTCCC16850.10538547944451532No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC16140.10094490434625979No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)