FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133879
Sequences flagged as poor quality0
Sequence length250
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC728906.428375514494933No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC420933.7123008716097576No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC397443.505135909563543No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC387353.416149342213764No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC349443.0818103166210857No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA316202.7886573435084343No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA299462.6410225429697527No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC249762.2027041686105835No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC215051.8965868492140696No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTCCCC174831.541875279461036No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA170061.4998072986623792No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA152151.3418539367957252No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA117601.0371477027090192No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG97250.8576752898677901No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC91210.8044068194225309No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTCCCC90140.7949701864131887No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTACCC81740.7208882076482588No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG73310.6465416503877398No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC60440.5330374757800436No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA60360.5323319331251395No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC57400.5062268548936879No Hit
AAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT46920.4138007671012516No Hit
AAGACCGATGGGCCCTTGGTGGAGACTGAGGAGACGGTGACCGTGGTCCC45300.3995135283394436No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG43890.387078339046759No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTACCC41230.3636190457711978No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA37220.32825372019413007No Hit
AAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG36570.3225211861230343No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC34170.30135490647591145No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC31810.28054139815624063No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC29700.26193271063314516No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC29530.26043343249147394No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC25840.22789027753402258No Hit
AAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT25520.2250681069144062No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG25460.22453894992322815No Hit
AAGACGGATGGGCCCTTGGTGGAGACTGAGGAGACGGTGACCGTGGTCCC23040.20319628461237926No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTTTGTTGCAGCTGC21040.1855577182397769No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC20380.1797369913368181No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC20300.17903144868191403No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA19580.1726815647877772No Hit
AAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG16530.14578275106955857No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC16020.14128491664454498No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC15900.1402266026621888No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC14580.12858514885627126No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC12450.10980007566944974No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC12250.1080362190321895No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC11530.10168633513805264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)