FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194296
Sequences flagged as poor quality0
Sequence length250
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC999908.372296315151353No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA624055.225254040874289No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA345122.8897358778728224No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGAGGTGGGGGTCTCAGCCCTTCCT304612.5505402345817116No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC299842.510600387173699No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC232351.9454975985852754No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTACCC213861.7906783577940475No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG205911.7241119454473597No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGAGGTGGGGGTCTCAGCCCTTCCT162601.3614715279964096No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC157431.318182427136991No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC153871.2883740714194805No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC139191.1654564697528922No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTCCCC138581.1603488582394985No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC123721.0359240925197772No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTACCC107070.8965114176050158No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA100970.8454353024710792No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG98860.8277679905149142No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC90330.7563451606636881No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC80750.676130540502522No Hit
AAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG71770.6009398005184644No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC71760.6008560691821793No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTCCCC70350.5890499507659743No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA69460.5815978618365967No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC64280.538225029640893No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC62390.5223998070830012No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTCCCT60840.5094214499588041No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC54680.4578429468071567No Hit
AAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT48930.4096974284432No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA42640.3570304179198457No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC37410.3132389290427164No Hit
AAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG34830.2916362442811497No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTCCCT32650.27338281297098876No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCACCACAGGCTCTGGCTGACTC32610.2730478876258482No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC30430.2547944563156872No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC30210.25295236691741413No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTATGTGCCCCTCCTGGATGAGCTC28860.24164863651891993No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA26600.22272535451847783No Hit
AAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT26100.21853878770422072No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGGGGCTGCTCGGTCCTGGTACCC24530.20539296790745343No Hit
AAGACCGATGGGCCCTTGGTGGAAGGGGAGAAGGTGGGGAGCCCGGACCT22980.19241461078325642No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA22670.189818939358417No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC19480.16310864308345668No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTAGCGAGACGGTGACCAGGGTTCC17600.14736715186184998No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC17520.14669730117156887No Hit
AAGACGGATGCGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC15570.13036969059596618No Hit
AAGACGGATAGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC14770.12367118369315479No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA14710.12316879567544395No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG14690.12300133300287366No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCACCACAGGCTCTGGCTGACTC14200.11889849752490171No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGCGGCTCTGCCCTGGTCCCC13830.11580043808235145No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCGGCGGCTCTGCCCTGGTCCCC13100.10968805053353607No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA12970.10859954316182922No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGTTCCTGGTACCC12370.1035756629847207No Hit
AAGACGGATGGGCCCTTGGTGGAAGGGGAGAAGGTGGGGAGCCCGGACCT12220.10231969294044356No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGGCTCCAGTGATG12080.10114745423245158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)