FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853154
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC73104918.972742849104915No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC3515139.122734258739722No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC2472636.417158514816693No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC1231983.1973287338113137No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC520951.3520092890136237No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGCTTCC419971.089938268753338No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGATGACCAGGGTCCC286750.744195534359644No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC250650.6505060529633646No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC225230.5845341245120231No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGCTTCC203930.5292547352117253No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAAATTCC156340.40574552691120047No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC150420.39038149007280787No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGATGACCAGGGTCCC141800.3680102067033916No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC139460.3619372597098377No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC112080.29087858933227173No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC94810.2460581642986499No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC92550.24019283942453376No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGTTCC91230.2367670744538111No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC86860.2254257161795246No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC85620.22220757332823965No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAAATTCC77450.20100416438066063No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC75630.19628076116345208No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC73890.19176498006568127No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC72620.18846897891960715No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC68830.17863288101124428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC58860.15275797437631614No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC56990.147904807334459No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC55930.14515381425190896No Hit
AAGACCGATGGACCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC51750.13430555851128712No Hit
AAGATCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC51360.13329340067902815No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC51320.13318958961930927No Hit
AAGACCGATGGGCCCTTGGTGGAGACTGAGGAGACGGTGACCAGGGTTCC48390.1255854294949021No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC46000.11938271867669965No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGTTCC45580.11829270254965153No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC45520.11813698596007322No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC44590.11572337882160952No Hit
AAGACGGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC42920.11138926707834672No Hit
AAGACCGATGGGACCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC42110.10928709311903961No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC41720.10827493528678064No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC41680.10817112422706178No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC41340.1072887302194514No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC40850.10601704473789525No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC40080.10401868183830701No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGATCC38720.10048910580786545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)