FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728982
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC55023820.16275666164159No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC29373710.763610753020723No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGACGACCATTGTCCC1055113.866313519107125No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC661132.4226249934957433No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGACGACCATTGTCCC577792.117236390712727No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGATCAGGGTTCC474161.7374977189296228No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC351711.2887956021696No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGATCAGGGTTCC256900.941376674525519No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCCGACCATTGTCCC108080.3960451186559677No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC75620.27709966573616096No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC71300.2612695869741904No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC70740.25921753972726824No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC62490.228986486536005No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGCCGACCATTGTCCC58090.21286325816733126No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC42070.15416004942502368No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC41940.15368368131413104No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC41740.15295080729737315No Hit
AAGATCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC41660.15265765769067No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC41070.1504956793412342No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC40690.1491032187093942No Hit
AAGACCGATGGACCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC40240.14745425217168892No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC39690.14543884862560472No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC39650.14529227382225315No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC38360.1405652364141647No Hit
AAGACGGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC37780.13843990176556678No Hit
AAGACCGATGGGCCCTTGGTGGAGACTGAGGAGACGGTGACCAGGGTTCC37580.13770702774880889No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC36430.133493002152451No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC35050.12843617143682148No Hit
AAGACCGATGGGACCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC32540.11923860252650988No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC31630.11590402575026146No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGTTCC30320.11110370094049724No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGCGACCATTGTCCC29450.10791569896760037No Hit
AAGAGGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC29190.10696296274581511No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC28100.10296879935448457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)