FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842786
Sequences flagged as poor quality0
Sequence length250
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA1607788.724724411841635No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA852694.627178630616903No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA795644.3175930357621555No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGGTGGTGCCATGTGA656773.5640058042550793No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGATGGCGCCATGTGA401612.179363203323663No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC302611.64213316141972No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCAAGAGAGGTGGTGCCATGTGA155860.8457845892035212No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC154180.8366679581893935No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC130430.7077870137932456No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC84410.4580564427991096No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC72390.3928291185194591No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC68950.37416173120481705No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC67640.3670529296402295No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACGAGAGGTGGTGCCATGTGA67000.3635799273491333No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCGTGGTCCC60080.3260280900766557No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGGGCCATGTGA58310.31642306811534276No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGCGTTCC50920.27632074478534135No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGACGAGACGATGACCAGGGTTCC46120.25027322760211984No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGAATTCC44920.24376134830631446No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC42200.2290010885691556No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC37960.2059924483906433No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCAAGAGAGCTGGCGCCATGTGA36920.20034881966761198No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTTCCATGTGA36280.19687581737651577No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC35650.19345708074621795No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCACGAGAGGTGGTGCCATGTGA35020.19003834411592013No Hit
AAGACCGATGGGCCCTTGGTGGACAAGACAGTTGGTGAGAGGCCAGCTCA34750.1885731712743639No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC32120.1743013024843905No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGGGCCATGTGA31360.17017711226371376No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCGTGGTCCC30830.16730103224139972No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTGCC30560.1658358593998435No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCACGAGAGATGGCGCCATGTGA28710.15579671215214355No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGCGCC28210.15308342911222464No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGCCCC28120.15259503816503925No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGCGTTCC27890.15134692796667654No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGCGACCAATGTCCC27710.15037014607230575No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACAGTGACCAGGGTGCC26990.14646301849482252No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCACGAGAGGTGGTGCCATGTGA26320.14282721922133118No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCATTGTCCC24990.1356098863351469No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGGTGGGGCCATGTGA24320.13197408706165556No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACACCTTCCCGGCTGTCCTAC23820.12926080402173665No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGAATTCC22600.12264039340433452No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGCGGTGGTGCCATGTGA22400.12155508018836696No Hit
AAGACGGATGGGCCCTTGGTGGAAGCAGAGGATACGGTGACCAGGGTTCC22080.11981857904281887No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGCGGTCCC21530.11683396769890807No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACACCTTCCCAGCTGTCCTAC20420.1108104793502881No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC20180.10950810349112702No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGC20030.10869411857915136No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCACTGTCCC18990.10305048985612003No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATGTGA18710.10153105135376544No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTTCCATGTGA18710.10153105135376544No Hit
AAGACGGATGGGCCCTTGGTGGACAAGACAGTTGGTGAGAGGCCAGCTCA18630.10109692606737843No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCAAGAGAGCTGGCGCCATGTGA18470.10022867549460436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)