FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305159
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGG821386.2933328429716235Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGAT623694.77865148997172Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGTCTCCCTCACTGCCCAG495843.7990773537936757Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACTCTGAGAGGGGCTGTGCT250741.9211452397753837Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATC196201.5032651194222313Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTC180411.3822836911058347Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGGTCTCAGAGAGGAGCCTT165961.2715692111076122Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGA119950.9190451125111959Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCA114560.8777474621865995Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGCACAGAAGTTCCAGGGCAGAGTC103710.7946158284163078Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGGCTGTGCTGAGAG83730.6415310318512917Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGTCTCCCTCACTGCCCAGC77460.5934909080043121Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGTCTCCCTCACTGCCCAGCT75280.5767879622329539Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCA72920.5587058741501993Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACTCAACAACCACATCTGTC70480.5400108339290461Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCC67310.5157226054450071Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTG67260.515339510358508Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGTCTCCCTCACTGGCCAG67070.5138837490298117Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGATCTCATGTGCAAGAAAA61460.47090048032461945Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTGAGAGGCGCTGCTGGGC57730.44232158687179113Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGTGCTGAGAGGCGCTGCTGG55010.42148121416624335Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGAAGAAGCC54170.4150452167130595Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCC48460.3712957578348692Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGACGAGCCCA48170.3690738063331747Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCAC45980.35229424154451683Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGCACAGAGGTTCCAGGGCAGAGTC43710.3349017246174604Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGTAGAAGCC38220.2928378841198659Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCTT37440.28686160077048084Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTA35600.2727637015873162Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGAGGTCTCAGAGAGGAGCCTTA33520.25682694598895617Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGATAGTGGGAGCACCTACTACA33510.25675032697165634Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGGCGCTGCTGGGCGTCTG32510.24908842524167554Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGTCACCACGCTGCTGAGGGA31960.2448743792901861Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGATCTCATGTGCAAGAAAAT31430.2408135713732963Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCC30850.23636966836990742Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTGGGAGAGCGATGGGGCT30340.23246209848761723Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGCTCCCTCACTGCCCAGCTGG30320.2323088604530176Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGCGGGATGGGCAGA30170.23115957519352048Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGACTCCTGGTTCTGGGTGC29280.2243404826538376Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACCCAACAACCACATCCCTC28880.22127572196184525Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCAG27920.2139202963010637Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGATGCCCGGGAAAGGCCTGGAGTGGATGGG25530.1956083511664096No Hit
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGAGGCAGTGGTCACAACTC24550.18809968747102843Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGAGGCGCTGCTGGGCGTC24370.1867205451596319Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGGAGACGAGCCCAGCACTG24320.18633745007313285Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGAGGGGCTGTGCTGAGAGG24110.18472845070983687Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGGTTACCGTGTCCAGCTAA23880.1829662133119413Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACCACTCAACAACCACATCT23640.18112735689674592Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGACTCTGAGAGGGGCTGTGCTG23230.1779859771874538Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGGCGGAGGACTCCTGGTTC22570.17292912204566646Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGA22480.1722395508899682Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCCAGCCTT22080.16917479019797588Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAG21590.1654204583502853Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGAGGTAAGCAGCTGCCCCA21260.16289203077939163Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCCAGCCCTG21060.16135965043339548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGCACAGAAGTTTCAGGGCAGAGTC20960.1605934602603974Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGGAGAACCAAGCCCTGAAT19720.15109270211522122Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGCTCTCAGAGAGGTGCCTT19380.14848765552702775Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGTCTCAGAGAGGAGCCTTAG19330.1481045604405287Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGCACTCTGAGAGGGGCTGTGC18670.1430477052987414Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGCCGCCAGGCTCCAGGCAAGGGA18660.14297108628144156Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGTGCTTTCTGAGAGTCATG18650.14289446726414176Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGTCTCCCTCACTGCGCAG18500.14174518200464464Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGTGCTCTCTGAGAGTCATGG18360.14067251576244733Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAAGCACCTGTGGTTCTTCCT18220.13959984952025Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGTGGATTGGGAGTATCTA17950.1375311360531552Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGACCCTGCCATGGGCACCAG17720.13576889865525962Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGAGATGAGATCCTGGCCTG17260.13224442385946847Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGGTCTGGGAGAGGAGCCCC17170.13155485270377018Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGATGAACCCCAAGAGTGGTAA17020.13040556744427306Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGAGGGAAATGCTTTCTGAGAG16380.12550195033708536Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGCAGGGAACCCTGGTCACCGTCT15650.1199087620741994Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGAGAGGTAAGCAGCTGCCCCAC15630.11975552403959977Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCTTAGCCCT15590.11944904797040054Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGATTGGGAGTATCTATTATA15260.11692062039950687Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGTAGAAGCA15250.11684400138220706Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTGTGCTGAGAGGCGCTGC15120.11584795415730956Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGATCGGCTGGGTGCGCCAGA15120.11584795415730956Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGCGAGTGGATGGGAATTATGTA15040.11523500201891111Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGCCCAGGACTGGTGAAGCCTTCG14960.11462204988051264Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGATCACACAACAGCCACATCC14800.11339614560371572Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGTCTGGGAGAGGAGCCCAGCA14760.11308966953451649Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCTGAGGGAG14750.11301305051721668Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGTGTGCTGAGAGGCGCTGCTG14720.11278319346531725Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGACAGTGACCCTGATCTGGTA14560.11155728918852033Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGATACTTTCTGAGAGTCCTGG14500.11109757508472148Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGTCATCTATCCTGGGAACTCTGA14440.11063786098092263Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTTTTCTGAGAGTCATGGA13950.10688352913323204Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGAGCACCTGCACTGTCTCTGGTGG13890.1064238150294332Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGACCTTCAGCAACTATGCTATCA13680.10481481566613723Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTCGGGGAGGGAGTTGGTTTGCAGATGT13500.10343567335474067Clontech Universal Primer Mix Long (100% over 21bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCCA13430.10289934023364203Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGGCAGTGGTATCAACGCAGAGTACGGGGGCTCTGAGAGAGGAGCCCAG13220.10129034087034608Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph