FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524092
Sequences flagged as poor quality0
Sequence length250
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC23548715.450970151408184Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCATCACCCAGCAACCACATCT986646.473624951774565Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGCCGCGTCTGTGAAAGACAGATTCACC928026.089002501161348Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGGCATGGATCTC589113.8653178417050937Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGCGGAGCCCCAGC565473.7102090949890165Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGGCATGGATC285761.8749524306931602Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTTCTGAGAGGCATGGATCTCA224371.4721552242253093Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGCGGAGCCCCAGCCCCAGA199771.3107476451552793Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAGCAACCACATCTGTCCTC172251.130181117675311Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCATCACCCAGCAACCACAT166221.0906165769520475Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACCCAGTACTTCGGGCCAGGCA161051.056694740212533Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAACCACATTCCTCCTCTACAGAA141070.9256002918458991Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGACACAGTGCTGCCTGCCCC139310.9140524325303196Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGCGGAGCCCCAGCC132700.8706823472598766Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCGCGTCTGTGAAAGACAGATTCACC124800.8188482060138101Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCAATGCACAGATACAGAAGAC122240.8020513197366038Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACCAAGCCCTGAATCAGGTGCA111120.7290898449699887Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAACTGGCAGACACCTGCCTGA109500.7184605653726941Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATTCCTGTATGGGGTGGTATTCC93910.6161701524579881Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCATGGGGTAGGATTCCTCT93780.6153171855767238Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCCAGAGCTGGAAACACCTCCA91360.5994388790178021Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGCATGTGCGTGTGTTGGCATG90790.5956989473076428Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCCTGCTCACAGTGATCCTGA89690.5884815352354057Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCATCACCCAGCAACCACATCTGT89130.5848072163622668Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCGGAGCCCCAGCCCCAGAA86770.5693225868254672Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCACAGTGACCCTGATCTGGT85800.5629581416344945Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGTCTCAGGATGACTTCCTTG85270.5594806612724167Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGCAGGTGGCAGCAGGGGAACG80290.5268054684362886Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTTCCCCAGGAGGACCAAGCCCTG79780.5234592137482513Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCATCACCCAGCAACCACATCTG79120.5191287665049091Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGACCAGATGCCTGAGCTAGGA70060.45968353616448354Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTTCTGAGAGGCATGGATCT63910.41933164139697604Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGCATCACCCAGCAACCACA63830.4188067387008133Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAACCACATTCCTCCTCTACAGAAG63240.41493558131661346Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGGCATGGATCTCATGTGCA62650.41106442393241355Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGCTTTCTGAGAGGCATGGATCT60860.39931972610577315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACACAAGATCCTGCCCTGGAGCT59060.3875094154421124Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACAACCACATTCCTCCTCTACA56510.3707781420019264Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCATCACATAACAACCACATTCC55790.3660540177364621Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGGCAGTGGTCACAACTCTCC55350.3631670529075673Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATAAGGGAAATGCTTTCTGAGAGG54720.359033444175286Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCATGTTCACAGAGGGCCTGGTCTG51980.3410555268317136Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCATGGACTGGACCTGGAGGATC51280.33646262824028994Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCTACTCCCTCAGCAGCGTGGT50570.331804116811846Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCATCACCCAGCAACCACATCTG47930.31448232783847696Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCTGAGGTGAAGA47650.3126451684019075Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAGCAACCACATCTGTCCTCT45480.29840718276849426Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTGCCCTGAGAGCATCACCCAGCA42660.2799043627287591Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGTGTTTTCATTTGGTGATCA34210.22446151544657406Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTGTTTTCATTTGGTGATCAG33590.2203935195513132Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGAGAGCGGAGCCCCAGCCCCA31950.20963301427997785Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAAGTTTCAAGACTGGGTCACCATT31230.20490889001451357Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGATCTCAAGAGATGATTCAAAGAATT29930.1963792212018697Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACCATTACCAGGGACACGTCCATC28880.1894898733147343Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATCACATAACAACCACATTCCTCC28260.18542187741947336Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC24920.16350718985468068Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTCTGGGACAGCAGAGCGGGC22750.1492692042212675Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC21520.141198825267766Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGACATCTGACGACACGGCCGT21340.14001779420139993Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGCTAGTCACCAAAAGGAGTCA20790.13640908816528136Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAGTGAAGGTCTCCTGCAAGGCTTC20760.13621224965422035Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATCACCCAGCAACCACATCTGTCC20690.13575295979507798Clontech SMARTer II A Oligonucleotide (100% over 23bp)
NCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC20000.1312256740406747Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGTCACCAGACTGAGAACCACCG18000.11810310663660722Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAACCCTGGTCACCGTCTCCTCA17610.11554420599281408Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACACGGCCGTGTATTACTGTGCTAG17560.1152161418077124Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCGTACTAGAAACAGAGCAAGCAGT16430.10780189122441426Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACAGAAGTTTCAAGACTGGGTCACC16380.10747382703931259Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCGTGTATTACTGTGCTAGAGTGGTC15330.10058447915217716Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)