FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406106
Sequences flagged as poor quality0
Sequence length250
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT22603716.075388341988443Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTG913736.498300981576069Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC384502.734502235251112Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA281101.9991380450691483Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGACTCCTGTGCCCCACCATG247961.76345168856402Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACCCAAAAACCACACCCCTCCT192331.3678200647746328Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAGAAACCACACCCCTCCTT182331.2967016711400137Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATACTTTCTGAGAGTCCTGGACC156281.1114382557218303Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTGGGA140560.9996401409282087Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACTCCTGTGCCCCACCATGGAC138380.9841363311158619Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCGCCCAGCAACCACATC128420.9133024110557809Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTGACTCCTGTGCCCCACCATGG109390.7779641079691005Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTAGAA108360.7706389134247347Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCAC91810.6529379719594397Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC86990.6186589062275533Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCATCACCCAGCAACCACATCT86430.6146762761840145Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAAATACTTTCTGAGAGTCCT85520.6082045023632642Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGCAAGAACATGAAACATCTGA83390.5930562845190903Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATACTTTCTGAGAGTCCTGGACCT83200.5917050350400325Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAGAAACCACACCCCTCCTTG80460.5722185951841469Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCATCGCCCAGCAACCACATCT79650.5664580052997427Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAAGAGCCCCAGC78830.560626297021704Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTTTCTGAGAGTCCTGGACCTC77050.5479672229547417Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCAGCAC74800.5319655843869523Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTGCAAGAACATGAAACATCTGAG68870.48979237696162314Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGACGAGCCCAGCA64870.4613450195077754Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGACTCCTGTGCCCCACCATGG64240.4568645607087944Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCTACTCCCTCAGCAGCGTGGT61890.44015173820465886Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAGCAACCACATCTGTCCTC59120.4204519431678693Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACATGGGAAATACTTTCTGAGAGT58590.4166826683052345Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACATGGGAAATACTTTCTGAGAG57660.4100686576972149Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTAGAAG54080.38460827277602117Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTGACTCCTGTGCCCCACCAT52410.37273150103903974Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTCTCCCTCACTGCCCAGCTG42790.30431560636253596Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGACGAGCCCAGCACTGGAA42420.30168422579805504Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGTGGAACTGAGTAGACTGAGTTCTGAC40600.28874067815655435Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGGGAAATACTTTCTGAGAGTC40010.2845446929321118Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGGGAGAGGAGCCCAGCACT39680.2821977859421694Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAAATACTTTCTGAGAGTCCTGGA38720.2753704201532459Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTGGGAT37950.2698943038433802Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAAGGTGGTGTGAGGCCATC35730.2541060204564947Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCTGGACCTCC33690.2395978681550324Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAAATACTTTCTGAGAGTCCTG31390.22324063761906998Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCTGAGGGAGTAG28980.20610110475312673Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCATCACCCAGCAACCACAT28610.2034697241886458Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCC27840.19799360787878012Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGACGAGCCCAGCACTGGAAG27810.19778025269787625Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATCACCCAAAAACCACACCCCTCC26090.18554788899272173Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGCTCCTGCTCACAGTGATC25720.18291650842824084Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT25640.18234756127916388Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCC24710.1757335506711443Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGACGAGCCCAGCAC23300.16570585716866296Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGATCACGGCTCCTCACCATGGA22190.15781171547522022Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTCTCCCTCACTGCCCAGCTGG21940.15603375563435473Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTGACTCCTGTGCCCCACCATGGA21790.15496697972983545Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT21600.15361573025077768Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCAGAGTCACCATGACCACAGAG21440.15247783595262376Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAAATACTTTCTGAGAGTCCTGG20190.14358803674829637Clontech SMARTer II A Oligonucleotide (100% over 23bp)
NCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT19440.13825415722569992Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGCTGCTGAGGGAGTAGAGTCCTGAGGA19360.13768521007662296Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGACGAGCCCAGCAC18810.1337736984267189Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGGGAGAGGAGCCCAGCACTGG18800.13370258003308427Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAGGGCAGAGTCACCATGACCACAGA18670.13277804091583423Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCACCCAAAAACCACACCCCTCCT18160.12915100284046865Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCGTCACATAACAACCACATTCC18100.12872429247866093Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCAAGAACATGAAACATCTGAGG17770.1263773854887185Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTCCTGCACCAGGACTGGCTGAATG17720.1260217935205454Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCCCCAAGCTAGGAGATCCTGCC17610.12523949119056457Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCATAAATACTCCACTGAAGTTCCAG17560.12488389922239147Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGGGCACAGGCTCGGGAGGAGG17530.12467054404148763Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCATATTACTGTGCACACAGACGCGT17450.12410159689241068Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCATCGCCCAGCAACCACAT16510.11741646789075647Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCTGGGAGAGGAGCCCAGCACTA16430.1168475207416795Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACCCAGACCTACATCTGCAACGTGA16250.11556738965625636Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGAGAGGCGCTGCTGGGCGTC16170.11499844250717942Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTCACCACTGCAGACCAGAAT16120.1146428505390063Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAGCAACCACATCTGTCCTCT15900.11307824587904466Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACCATGGACGGGACCTGGAGCATC15780.11222482515542925Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCCAGCCCTGGGA15670.11144252282544843Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGCATCACCCAGCAACCACAT15460.10994903655912143Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCCCACCTCGGTGAGTGGGGTC15120.10753101117554438Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCATCATGGGGTCAACCGCCATCCT15100.10738877438827513Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAACCTCCTGTGCAAGAACATGA15010.10674870884556356Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCGCAGCAACAGGTGCCCACTCCCAG14990.10660647205829432Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACCTTCCCGGCTGTCCTACAGTCC14780.10511298579196733Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACCCAAAAACCACACCCCTC14620.1039750914938134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCTCCTGTGCAAGAACATGAAA14590.10376173631290955Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAATACTTTCTGAGAGTCCTGGAC14370.10219713165294793Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACATGGGAAATACTTTCTGAGA14360.10212601325931331Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGCATCACCCAGCAACCACA14310.10177042129114022Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCACATAACAACCACATTCCTC14210.10105923735479402Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCACGAACTACAACTCGTCTCTCAA14120.10041917181208244Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)