FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451052
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG108750.749456256564203Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCTGAGGGAGTAG87290.601563555268867Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTCTGAGAGGGGCTGTGCTGAG85190.5870912965214203Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCACAGACACAGTGCTGCCTG70670.48702596461050324Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGGACCAAGCCCTGAGCACA68840.4744144248448712Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGACACAGTGCTGCCTGCCCC67210.4631811954361387Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGATGAGATCCTGGCCTGGA66910.46111372990078925Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAAGGTGGTGTGAGGCCATC65700.4527749522415462Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGACACAGTGCTGCCTGCCCCTT63840.4399566659223791Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCTACTCCCTCAGCAGCGTGGT59070.40708396391032164Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGACACAGTGCTGCCTGCCCCTTT57910.3990897638403035Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGAAGGTGGTGTGAGGCCATCA57730.39784928451909374Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGCAGTGGTCACAACTCTCCC54530.3757963188086988Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGAGAA54270.3740045153447292Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAACCCTGGTCACCGTCTCCTCA52210.35980791866866246Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGGCTGTGCTGAGAGGCG50390.34726529442087534Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGTGACCCTGATCTGGTAAAG46910.32328269421082084Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTTTCTCAGGGGAGAGGCCATC45340.3124629579091583Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGGCAGTGGTCACAACTCTCC44500.3066740544101797Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGATGAGATCCTGGCCTGGAC44370.30577815267819486Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGTTCACAGAGGGCCTGGTCTG38730.2669098006136238Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTTGCTCACAGTGACCCTGA38150.2629127005786147Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCGGTCACCGTCTCCTCAGCCTCCAC36970.25478066947290656Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTCACAGAGGGCCTGGTCTA36580.25209296427695216Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGGATC35410.244029848689089Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCTGGTAAAGCTCCCATCCTG34850.2401705796897699Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAACTGGCAGACACCTGCCTGA33370.22997108304871225Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGGTGGGTACTGGAGAAG32040.22080531917532936Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCACAGATACAGAAGACCCCTCCG31070.21412051394436588Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC29700.20467908799960305Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGATACAGAAGACCCCTCCGT29610.2040588483389982Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTCAGAGAGGAGCCTTAGC27630.19041357580569132Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTTGGAGAGGGGTGGGTACTG27630.19041357580569132Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCACCACGCTGCTGAGGGAGTA26830.18490033437809258Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATTCCTGTATGGGGTGGTATTCC26680.18386660161041782Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACAGTGACCCTGATCTGGTAAAGC26620.18345310850334792Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGATCCTGATCTGGTAAAGCT26610.18338419298550293Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGAACCAAGCCCTGAATCAG25040.1725644566838404Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCCTGATCTGGTAAAGCTCCC24930.17180638598754558Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAAACCACCTGGAGCCCCCAGA24450.16849844113098633Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGACACCTGCCTGATGCTGCCATG24350.16780928595253652Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGATGAGATCCTGGCCTG24220.1669133842205517Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTGCAGTGCTGCCTGCCCCAC24070.16587965145287695Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA22900.1578165358650138Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGCTGAGAGGCGCTGCTGGGCG22360.15409509790138465Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC20970.14451584092093187Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT20840.14361993918894705Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGATCTC20760.14306861504618718Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCTCCAGC20590.14189705124282245Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGCAGACACCTGCCTGATGCTGC20440.1408633184751477Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGACGTTACAGAAACCACCTG18890.13018141320917512Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGACCCGAGGCTAGCATGGGCTG18580.12804503215598062Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCTGGAAACACCTCCATCCT18320.12625322869201103Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACTCTGTCATGGGCACCAGGCT18170.1252194959243363Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAAATAAGAAGTCTTTGAAATGTGA18090.1246681717815764Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACATAACAACCACATTCCTC17790.12260070624622688Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGATGCAGTGCTTCCTGTCCCTC17360.11963733897889255Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCTTTCATCAATGCACAGATACA17230.11874143724690776Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTCAGAGGCCCCATCTCAGACC17150.11819011310414788Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTTTGCTCACAGTGACCCTGAT17080.11770770447923298Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACGGTCACCGTCTCCTCAGCCTCCA17060.11756987344354303Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCATGGGGTAGGATTCCTCT16900.11646722515802328Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGAGAGGCGCTGCTGGGCGTC16780.11564023894388346Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACGTCTGGGGCCAAGGGACCACGGT16690.11501999928327862Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTGGAAATGGTGACCTCACAGG16650.11474433721189868Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGGCCCCATCTCAGACCCGAGG16240.11191880098025432Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGGACCAAGCCCTGAATCAG16030.11047157510550966Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTAGAGTCCTGAGGACTGTAG15560.10723254576679539Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGGAGTAGAGTCCTGAGGACTGT14690.10123689571428177Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)