FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316010
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG3180429.591104972542302Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGCCCCATCTCAGACCCGAGG1706155.1451895500918265Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCTGAGCACAGACACAGTGCTGCCT1224793.69356545969403Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCACAGACCCAGAAGACCCCTCC1143323.4478786252152434Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGGCCCCATCTCAGACCCGAGG1122293.3844590335976066Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTTCACAGTGACACTGATCTGGT1097653.310152864436476Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAAGGTGGTGTGAGGCCATC1043763.147638276121001Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCAGAGGCCCCATCTCAGACCC1033073.115400737633481Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCTCAGACCCGAGGCTAGCATG1011743.051076444280928Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTCACAGAGGGCCTGGTCTA1004333.0287303114284936Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGTTGCTCACAGTGACCCTGAT998833.0121441129550273Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGGGAGAGGCCATCACTTGAAG974132.9376570034469136Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAAGACCTATTCTTTCTTCAAA963952.906957457908752Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGATGAGATCCTGGCCTGGAC934522.818206217713457Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGCTGCTTCTGCTGCTTCTGG823422.4831650085494315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGGATACCTGGCTCGTATGCTGG762112.2982741306570245Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGAAGAAGCCTGT679692.049722407351003Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGCTCAAAATGCCCCTCCTT651291.964077309778921Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAGATCACGCAGATACTGGAGTCTCC570471.7203506623924538Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTCAAAGCAGCCATGGGAATCA544001.6405258126483333Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTCTGTTCACAGTGACACTGA531571.603041004098299Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCATGCTCACAGAGGGCCTGG504311.5208337731188988Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGTCTCCGCAGATCACACAGGT491931.4834997481913506Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCAGTGACATCACAGGAAA394821.1906477965989246Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTGTGTAGCAATCAGCCCCAGC292720.88274763948239Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCAGGTCCAGTGAATGCTGGTGTC246580.7436045126522538Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACAAAGAGGGGACAGAATGT82100.2475867081221106Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTGGGCATTATGGCAGGCACCAGA81350.24532495378481972Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGCCTTTTCTCAGGGGAGAGG70770.21341913926676942Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTCTGGGACAGCAGAGCGGGC68820.20753857798981307Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTACTTCGGCGCCGGGACCCGGC60810.18338304166754624Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCGGGGAGCTGTTTTTTGGAGAAGG50690.15286443647636766Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAAACATCCTGAGGACAGTGCCTGGA47650.14369679222921522Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)