FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927943
Sequences flagged as poor quality0
Sequence length250
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGTGTCAAGTCCAGTTCTACGGGCTCT2983667.595985990631737Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGCAGTGGTCACAACTCTCCC2900727.3848322137057485Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGC2470536.289627929936866Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGATCCTGATCTGGTAAAGCT1829714.658188777179302Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACTATGAGCAACCAGGTGCTCTGCT1700034.32804142015299Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG1672054.256808207247407Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAATGCACAGATACAGAAGACCCC1641904.1800504742558635Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCACAGTGACCCTGATCTGGT1575814.011794468504252Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTCATCCCTCCTCGCTGGTGAA1574854.009350441185119Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCCCTGCCTGTGGGCTCCCTCCA1567913.991682160357215Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCACAGTGACACTGATCTGGTA1520653.8713647321256954Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGCCTGGTCTGGAATATTCCACA1487313.7864857000216143Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCTGGTAAAGCTCCCATCCTG1255813.1971186954596846Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTTCCTTTTTCTCATGCTTGTAAGC1102882.807780051798104Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACTCCAGGATCATCCACTGAGCAC942202.3987109792581003Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGGACCAAGCCCTGAATCAG819612.0866137823283077Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTATTTCCACAGATCACACAGGT459101.168805148139879Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCCCTCCTCGCTGGTGAATGGA399101.0160534406940223Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAAATGTGGACCATCCCATCAGAT171930.4377100176861019Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGCCATGAAAATATGTTCTGGTATCGAC163580.4164520717332202Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGTCCTGTCTCCTCATCTGCA131580.3349844944287634Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCACGTGGTCGGGGTAGAAGCTCCA78330.1994173540705657Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATATCAGACCCGAG55030.14009877434575807Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCAAGTCCAGTTCTACGGGCTCT53880.13717103328637917Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCGGCTCACCGTGCTAGAGGACCTG44850.11418190131577774Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)