FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723182
Sequences flagged as poor quality0
Sequence length250
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGATGGGGCTCTCAGCGGTGGGAA22751613.203248409047912Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGCAGTGGTCACAACTCTCCCC17662210.249758876311382Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGTCTAGGATTTGGATCTTGTTTTTTT1589849.22618736732394Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCCTGATCTGGTAAAGCTCCC1314237.6267625822461005Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCACAGTGACCCTGATCTGGT745684.327343252192746Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGAAGGTGGTGTGAGGCCATCA707404.1051960849173215Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAACATATGGGGCACAGGGCTATGT240131.39352662690302Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTGGGCAGGTCCAGTGAATGCTGGT193971.1256501054444625Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTACACCTTCGGTTCGGGGACC165800.962173467457297Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCATTTTGGTGATGGGACTCGA155270.9010655868039477Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAGGCTGTGAGCACAGATACGCAGT145220.8427432505678449Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCCTGTCTCCTCATCTGCAAAA121570.7054971558430856Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGATGCACCAAGCAAGGCTTTGAAAAAT119690.694587106875536Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAAAAGGACGGGAGAGAAAGTTTTT115270.6689368853667227Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCGCGTAGCAATCAGCCCCAGCA107540.624078013813979Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCACAGATACGCAGTATTTTGGCCC89400.5188076477121976Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTCTGCTCTCTGTCCTGTCTCC80380.46646262553810336Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCACAACTCCCTTTTCTGGTACAGA66110.38365071130037337Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCGGGCAGCACCGGTTTTTG59730.346626183421136Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTCGGGCCAGGGACACGGCTCA57760.3351938448753527Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTCTGAGAGGGGCTGTGCTGAG57650.33455549094640036Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAATCAGCCCCAGCATTTTGGTGA57570.3340912335435259Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAACCCAGCTCTCTGTCTTGGAGGAC53580.3109363955751627Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGCACCCGGCTGACAGTGCTCGA53220.3088472372622277Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGCACGTGGTCGGGGAAGAAGCCTGTGG51870.3010128935887213Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTGCCGTTCCCTGGACTTTCAGGCC49770.28882613676326707Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAGAGCAACCCTAGCCCCATTACCT49540.28749139673000296Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCAGCCAAGATCTTCGGCGGGGGTC48620.2821524365969468Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTAAGGAGCTCAAAACATC45530.26422049441092116Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCAAAACATCCTGAGGACA44370.2574887620692417Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAAGGCTCTAGGCTGACCGT42530.24681084180312932Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGTAAGCAGCTGCCCCACTCT41830.24274858952797787Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGTGCCTGGAGAGGACCTGAA41180.238976498129623Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCTCTTCCCTTTCCAGAGGACCTGA39710.23044576835180497Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGGTCGGGGAAGAAGCCTGTG38480.2233078107826103Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAACATCCTGAGGACAGTGCCTGG34720.20148771284751116Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTAGGATTTGGATCTTGTTTTTTT32850.1906356960553209Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGACACGGCTCACCGTGCTAGAG31990.1856449289744206Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTGTGTAGCAATCAGCCCCAGC29220.16957001639989275Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACTCTGAGAGGGGCTGTGCTGAGA28510.165449731949382Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCATGCTGATGGCAACTTCCAATGAG26080.1513479133370706Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGGTCGGGGAAGAAGCTCCCT24130.1400316391420059Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCGGCTGACAGTGCTCGAGGACCTGA22110.12830913971942604Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCTGAGGGAGTAG21610.12540753095146073Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGTGCTGAGAGGCGCTGCTGG21350.12389869439211876Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTACCAGGACCAGACAGCTCTCAG21250.1233183726385257Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAACAACCACACCCCTCCTA20880.12117118215023137Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCAGTATTTTGGCCCAGGCACCCGGC20730.12030069951984178Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGGCTGTGCTGAGAGGCG20510.11902399166193706Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGTGCTGAGAGGCGCTGCTGGG19640.11397519240567741Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGAGAA19220.11153784104058655Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTGCCCCACTCTGAGAGGGGC18670.1083460713958247Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCCAGC17650.10242678950917547Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTGATCTATTTCTCATATGATGTT17640.10236875733381616Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)