FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434559
Sequences flagged as poor quality0
Sequence length250
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGACAGACACAGTGCTGCCTGCCCC1700336.9841396326809075Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG1497276.1500666034382405Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCTGGTAAAGCCCCCATCCTG1458405.990407297584491Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGGCCCCATCTCAGACCCGAGG1365495.608777606129077Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGCAGTGGTCACAACTCTCCCCA1356955.573699384570265Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTCACAGAGGGCCTGGTCTA1331915.470847081545363Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTTTCTTCAAAGCAGCCATGGGAA1292995.3109823996871715Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGAAGGTGGTGTGAGGCCATCA1275815.240415204560662Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTTGCTCACAGTGACCCTGA1200864.9325565739010635Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAAGGTGGTGTGAGGCCAT1048104.305091805127746Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGCCCCCAGAACTGGCAGACA799713.284824890257332Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTTTGCTCACAGTGACCCTGAT592892.4353075854805737Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAACTGGCAGACACCTGCCTGA545602.241062960478674Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAAGGTGGTGTGAGGCCATC530372.1785054295254294Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCAGTGACATCACAGGAAA494712.0320312631569Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGCGATGGGGCTCTCAGC207580.8526390200442873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTCACACATCCCTCCAGCTAGG184700.7586589604113106Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGAGAA149650.6146903812969823Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCATGATACTATGTATTGGTATAAA121360.4984886379833063Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATATCTGATCAAAACGAGAGGACA115390.4739667430528486Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCTTTTTCTCATGCTTGTAAGCT81400.33435213523270535Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCAGAAAAACAGAAGAGAAGCAA74090.3043261633831836Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCCTGAGGACAGTGCCTGGAGG29210.11998066179542168Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCAAAACATCCTGAGGACA27650.11357293045680962Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAAAACATCCTGAGGACAGTGCCTG26620.10934218476529015Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)