FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558811
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG47332413.300060048145292Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCTCAGACCCGAGGCTAGCATG1612384.530670496410178Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGCAGTGGTCACAACTCTCCC1437034.037949753442933Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGACCCGAGGCTAGCATGGGCTG1376953.8691293243726625Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGGCCCCATCTCAGACCCGAGG1374633.8626102931568997Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCAGGCTGCTCTGCTGTGCGGTTCT1259973.5404240348813127Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTCACAGAGGGCCTGGTCTA1172183.293740521764151Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTCTGTCATGGGCACCAGGCTC986832.7729205063151707Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGTGACCCTGATCTGGTAAAG762922.1437496961766165Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCCCCAAGCTAGGAGATCCTGC720352.0241310932218655Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACACAGTGACACTGATCTGGTAA690361.9398613750491387Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGACCCTGATCTGGTAAAGCT669771.882004973009244Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTTGAGGGTGAATTTACAGAGTT663521.8644429277081587Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAAGAGGTGGAGACGTTACAGAA627811.764100425675879Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACAGACCCAGAGGACCCCTCCATC607391.7067217112681734Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACATTAGGCCAGGAGAAGCCCCC592041.6635893280087084Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGGCAGTGGTCACAACTCTCC579061.627116472327415Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGTAGAAGCCTGT563461.5832816072559066Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCACAGTGACCCTGATTGGGC544401.5297243939057175Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACACAGTGCTGCCTGCCCCTTTG481531.353064267813042Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAAGACCTATTCTTTCTTCAA451281.2680639685557902Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCAGAGGCCCCATCTCAGACCC437031.2280225052693161Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCACAGTGATCCTGATCTGGTA400921.1265560323377668Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCCTGGGAGCAGTTCCCATAGACACT400621.1257130541633147Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCTGGTAAAGCTCCCATCCTG362931.019806896179651Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGGACCAAGCCCTGAGCACA352270.9898530717141203Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGGGCACCAGGCTCCTCTTCTGG341390.9592810632539913Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTTCCAAGACTCTCTCCGGTGGC338290.950570288784653Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGTTGCTCACAGTGACCCTGA329450.9257305319107983Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTTGAGGGTGAATTTACAGAGT284370.7990590115631315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCAGACACCTGCCTGATGCTGCC218280.6133509197313373Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCCCTCCTCCTTTGCTGTTGCT207900.5841838748952951Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAAGTTACCCAGACACCAAAACACC139120.3909170787659137Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGTGACAAATAAGAAGTCTTTGAAATG110470.3104126631057395Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGACACAGTGCTGCCTGCCCCTTT96240.27042739836422897Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGGTCGGGGTAGAAGCCTGTG83780.23541570485198568Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAAGGACCCGAGCTGAGTCTG75200.21130652906265604Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)