FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5417101
Sequences flagged as poor quality0
Sequence length250
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG59024410.895938621044724Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGAAGGTGGTGTGAGGCCATCA2762235.09909266967701Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGGCAGTGGTCACAACTCTCC2299904.245628796657105Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGCTGCTCACAGTGACACTGA1849343.413892412196117Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCAGACCAGAATCCTGCCCTGGGC1789783.303944305265861Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGTTCACAGAGGGCCTGGTCTG1534182.8321052164248Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGAACCAAGCCCTGAATCAG1522022.8096577855941764Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCAGCCCCAAGCTAGGAGATCCT1477432.7273443858624753Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCACAGTGATCCTGATCTGGTA1471882.7170990535343535Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCCTGCTCACAGTGATCCTGAT1466382.7069460214974765Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAACTGGCAGACACCTGCCTGA1431602.642741938907914Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGTCCTGTTCCCCTTTCATCAA1373912.536245862870196Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGCAGTGGTCACAACTCTCCCC1347152.4868467469962257Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGCAGTGGTCACAACTCTCCC1313002.423805648076342Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGACACCAGCCCCAAGCTAGG1302492.404404126856782Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGCCCAGCACCTCGCCCAAA1269692.3438551358004953Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGATGAGATCCTGGCCTGGA1212542.238355902908216Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGTTGCTCACAGTGACCCTGAT1109282.047737341430407Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGCCTTGCCTGGTCTGCCTCAC1102372.0349814411804394Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCATCTCAGACCCGAGGCTAGCA1095442.022188620813974Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTGTGGGGCCTTTTCTCTCCTG1090362.0128109112235495Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGCAGTGGAAACCACAGCCTAGT959861.7719071510758244Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGGACCAAGCCCTGAGCACA785451.4499452751573212Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACACAGATAGCCAGCTGCCTGTG318080.5871775327799869Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACACAGTTATGTTTTTTGGTACC258570.4773217261409747Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGATCCTGGCCTGGACCTGAAA243330.4491885973697002Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAAATTGAAATGACTCTGTCCCA203080.37488686291800727Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTCTCCCAGTCCCCCAGTAACAA177810.3282382957231183Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGACAGGTAGAGACTCCAGGAT167720.30961209695001074Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGAGAA93460.17252770439391843Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAAAAACATTCAGTACTTCGGCGCCG89260.16477447992939398Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGCTCAAAACATCCTGAGGAC66120.12205790514151389Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCACCACGCTGCGAGGGAGTAGA58240.10751137924140605Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGTCCAATTTCAGGTCATACTGCCCTT56650.10457622997983608Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)