FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434955
Sequences flagged as poor quality0
Sequence length250
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTGGGA28252311.602801694487166Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACAGGGCAGGAGCACAGGCTCAG2204129.05199480072527Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCCAGTCTTGGG1727057.092738880184644Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA1591466.535890807016967Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTG1477406.067463259074603Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGACCCCTAGATCACAGC1033474.244308416377305Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGCTGAGCTGGGTTTTCCTCGTT1003254.1201993465998346Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATACTTTCTGAGAGTCCTGGACC855503.5134119521716007Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTCTGAGAGTCATGGATCTC846383.4759574612261828Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCATGGACTGGACCTGGAGGGTC806253.3111494873621896Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCCAGC745763.0627260052033813Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCAGAGCCTGCTGAATTCTGGCT673572.766252353739597Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGCTCGGGAGGAGAAGGACAGA602862.4758568433502877Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGGATC530252.1776583140140167Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCCCTTGAGTTCTGGGGCCAG43450.17844272276079023Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)