FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728982
Sequences flagged as poor quality0
Sequence length250
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC45766416.770502700274314Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCC28874410.580648754737114Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGTCCTGGACCTCCTGTGCA27873110.213735378247273Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCCAGCCCTGAGAT2209628.096865424542925Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTAGAAG2094157.673740610967752Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCCTGGGAGAGGAGCCCAGCAC1996517.315951515986548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCCAGC1571335.757934643760933Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGCACTGGGTGCGACAGGTCCCT67890.2487740849884682Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACTATTAGTGTTAGTGGTGATACCA31180.11425505921255619Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC30520.11183657495725513Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC28850.10571707691732668Clontech SMARTer II A Oligonucleotide (95% over 23bp)
CCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC28120.10304208675616035Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGGCCCCATCTCAGACCCGAG27300.10003730328745299Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)