FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264018
Sequences flagged as poor quality0
Sequence length250
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCAGCAC1916264.494024180948579Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGACCTC1854474.349113910869982Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT1688583.9600677107835853Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGCTTTCTGAGAGTCATGGAC1475593.4605623146994215Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA1087882.551302550786606Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGTTTCCATTCAGTGATCAGC851111.9960281593558Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCC734771.72318690962374Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGTCTGGGAGAGGAGCCCCAGCC665251.5601481982486942Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAAGAGCCCCAGC599791.4066310226645384Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTCAGAGAGGTGCCTTAGC591851.3880100881375266Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACTCCTGTGCCCCACCATGGACA574541.347414574703953Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTAGAGAAGCCCCTGACGGCAC549061.2876587293956077Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACACAACAGCCACATCCCTC538231.262260149933701Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCCCCAGCCCTGGGATTCCCA525081.2314206928770002Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACCTCCTGTGCAAGAACATGAA506971.1889490147555664Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAACCACATTCCTCCTCTAAAGAAG505031.1843993153875054Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGCTTTCTGAGAGTCATGGACC474921.1137851669481695Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCT465881.09258450597535Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCCAGCCCTGAG450861.0573595139607759Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACTGAACACAGAGGACTCACCATG442081.0367686065115111Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACATAACAACCACATT429981.0083916156076265Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTGGTTTCTGAGAGTCATGGACC420350.9858072831775101Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCATCATCCAGAAACCACATCCCT418340.9810934193992614Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC416700.9772472817891481Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGGATC392680.9209154370361476Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTCCACCATGGACATACTTTG389480.9134107782847071Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCACATAACAACCAGATTCCT388720.91162842183124Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACATAACAACCACATTCCTC375110.8797101700790193Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC371390.8709860042804697Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATCACACAACAGCCACATCCCTCC370090.867937236662697Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTGGGAGAGGAGCCCAGCACTAGA349060.8186175574305737Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTGGGAGAAGAGCCCCAGC341280.8003718558411338Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTCTCCCTCACTGCCCAGCTGG341080.7999028146691688Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGGGAGAGGAGCCCCAGCCTTG336650.7895135527101433Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGAGTGTTTGCATTCAGTGATCAGGAC335380.7865351412681654Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCCAGCC335290.7863240727407812Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGTTTCCATTCGGTGATCAGCACT321630.7542885606955694Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCATCACATAACAACCAGATTCC312740.7334396806017236Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTGCTTTCTGAGAGTCATGGACCT297840.6984961132903286Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTGGAAG294440.6905224133669229Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCA289110.6780224661340548Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTGGAA286970.6730037255940289Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTAGAAG267700.6278116086751979Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCCAGCCCTGGGAT262760.6162262917276615Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGTGGAGCCCCAGC262640.6159448670244826Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCC258490.6062122627062081Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCCAGC255780.5998567548260818Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCTTAGCCCTGGAT255700.5996691383572959Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTGGGAGAGGAGCCCCAGC243240.5704478733438743Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCTTAGC243130.5701899006992934Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGTGGAGCCCCAGCCTTGGGA240770.5646552148701061Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCCCCAGCCCTGGGATTCC238090.5583700631657746Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCACGTGGTCGGGGTAGAAGCCTTT211380.49572961464984433Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGTGGAGCCCCAGCCTTGGG206220.4836283524131465Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCTTAGCCCTGGA205880.4828309824208059Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTGGGA202820.47565465248974087Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTGGGAT202750.4754904880795531Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCTGGGAGAGGAGCCCCAG201950.473614323391693Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCCCAGCACTGGAAGTCGC187010.43857694784590495Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTCAGAGAGGAGCCTTAGC182700.42846911059005843Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGGGAGAGGAGCCCAGCACTGG180920.4242946441595697Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCAC177760.4168837936425221Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGTCTCAGAGAGGAGCCTTAGCC168180.394416721505397Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGTTTTCTGAGAGTCATGGACCT166350.390124994781917Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGTGGAGCCCCAGCC142890.3351064653104185Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGCGGAGCCCCAGCCCCAGA141170.33107271123151916Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCCAGCCCTGGGA115310.2704256876964403Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCCCCAGCCCTGGGATTCCCAG100860.2365374630219666Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCCAGCCGTGAG67500.15830139553819894Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)